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Fig. 6 | BMC Complementary and Alternative Medicine

Fig. 6

From: Inhibition of human cytomegalovirus immediate-early gene expression and replication by the ethyl acetate (EtOAc) fraction of Elaeocarpus sylvestris in vitro

Fig. 6

Effects of the EtOAc fraction of ESE on HCMV MIE enhancer/promoter. HEK293 cells were transfected with (a) HCMV MIE enhancer/promoter-driven firefly luciferase or (b) NF-κB-dependent promoter-driven firefly luciferase plus control Renilla luciferase plasmids. Cells were treated with DMSO, ESE or the EtOAC fraction of ESE at concentrations of 5, 10, 25 or 50 μg/ml, and luciferase activity was determined using a dual luciferase assay system. MIE enhancer/promoter- or NF-κB-dependent promoter-driven luciferase activity was expressed in RLU by normalizing firefly luciferase activity with constitutive Renilla luciferase activity. To calculate relative luciferase activity, MIE enhancer/promoter- or NF-κB-dependent promoter-driven firefly luciferase activity in the presence of DMSO was set as 1. Data represent the average of three independent experiments. (RLU, relative luciferase light unit) Significant difference between samples was determined based on P values obtained from Student’s t test (* P < 0.01)

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