Fig. 5

Downregulation of HCMV lytic gene expression by the EtOAc fraction of ESE. HFF cells were infected with HCMV-Towne at an MOI of 1 and treated with either DMSO or the EtOAC fraction of ESE (50 μg/ml). At 0, 24, 48 or 72 h after infection, equal amounts of cell extracts were subjected to western blot analysis with antibodies against HCMV IE (IE1-72 kDa and IE2-86 kDa), ICP 36, UL83 and tubulin