Fig. 7

The effects of various Chinese herbal extracts (CHE) on the gene expression within the BDNF autoregulation loop using HUVECs. Cultured HUVEC cells with/without/with a given CHE (1 μg/mL for each herbal extract) pretreatment were lysed and the protein and mRNA gene expression levels of BDNF and TrkB were analyzed by Western blot (a) and real-time PCR (b), respectively. Data are expressed as mean + SEM. *, p < 0.05 compared to vehicle group by repeatedly measured one way ANOVA; #, p < 0.05 compared to vehicle group by Student’s t test (n = three to five independent experiments for each group)