Evaluation of antimycobacterial, leishmanicidal and antibacterial activity of three medicinal orchids of Arunachal Pradesh, India

Table 5 In vitro leishmanicidal activity against promastigotes and intracellular amastigotes of L. donovani

S.No.	Extracts and fractions	IC₅₀ ± SD(μg/ml) promastigotes	IC₅₀ ± SD (μg/ml) amastigotes
1	RrR Et₂O	56.04 ± 0.02	18.42 ± 0.26
2	RrR n-BuOH	500	125
3	RrR Aq ext	1000	1000
4	RrS Et₂O	500	500
5	RrS n-BuOH	1000	500
6	RrS Aq ext	1000	500
7	RrL Et₂O	500	250
8	RrL n-BuOH	1000	500
9	RrL Aq ext	300	250
10	TcR Et₂O	250	100
11	TcR n-BuOH	1000	1000
12	TcR Aq ext	500	200
13	TcS Et₂O	>1000	500
14	TcS n-BuOH	1000	550
15	TcS Aq ext	500	500
16	TcL Et₂O	>1000	500
17	TcL n-BuOH	>1000	500
18	TcL Aq ext	>1000	>1000
19	SnP n-Hex	76.32 ± 2.30	23.80 ± 0.73
20	SnP DCM	1000	500
21	SnP EtOAc	500	200
22	SnP Aq ext	500	500
23	SnF n-Hex	65.64 ± 0.22	22.16 ± 0.99
24	SnF DCM	1000	250
25	SnF EtOAc	500	250
26	SnF Aq ext	500	500
27	SnL n-Hex	500	200
28	SnL DCM	1000	500
29	SnL EtOAc	>1000	500
30	SnL Aq ext	1000	1000
	Amphotericin B	0.055 ± 0.5	0.25 ± 0.48
	Miltefosine	8.11 ± 0.36	4.37 ± 0.51

The effect of different plant fractions on promastigotes (Log phase; 1 × 10⁶ cells/mL) and intracellularamastigotes were evaluated by MTT based colorimetric assay, efficacy was expressed in IC₅₀ values
Standard deviation (SD) was calculated for IC₅₀ < 125 μg/mL against promastigotes and amastigotes

ISSN: 2662-7671