Evaluation of antimycobacterial, leishmanicidal and antibacterial activity of three medicinal orchids of Arunachal Pradesh, India

Table 4 Minimum inhibitory concentration (MIC in μg/mL) of the antimycobacterial activity of crude extracts/fractions of three orchids against H37Rv and MDR strain

S.No	Extract/Fraction	MIC values against H37Rv (μg/mL)	MDR Strain-
S.No	Extract/Fraction	MIC values against H37Rv (μg/mL)	Tb-14,348/16 (μg/mL)
1	RrR Et₂O	62.5	125
2	RrR n-BuOH	250	500
3	RrR Aq ext	>1000	>1000
4	RrS Et₂O	125	125
5	RrS n-BuOH	>1000	>1000
6	RrS Aq ext	500	500
7	RrL Et₂O	500	500
8	RrL n-BuOH	>1000	>1000
9	RrL Aq ext	>1000	>1000
10	TcR Et₂O	125	104.16
11	TcR n-BuOH	>1000	>1000
12	TcR Aq ext	250	250
13	TcS Et₂O	500	>1000
14	TcS n-BuOH	>1000	>1000
15	TcS Aq ext	1000	>1000
16	TcL Et₂O	375	>1000
17	TcL n-BuOH	>1000	>1000
18	TcL Aq ext	500	>1000
19	SnP n-Hex	250	500
20	SnP DCM	500	500
21	SnP EtOAc	125	250
22	SnP Aq ext	>1000	>1000
23	SnF n-Hex	15.7	62.5
24	SnF DCM	500	>1000
25	SnF EtOAc	125	250
26	SnF Aq ext	187.5	250
27	SnL n-Hex	125	250
28	SnL DCM	500	500
29	SnL EtOAc	>1000	500
30	SnL Aq ext	>1000	500
	Rifampicin (positive control)	0.08	1

Experiments were carried out in triplicate and results are expressed as mean of three replicate experiments. All crude extracts or fractions were dissolved in 0.2% DMSO and distilled water. Anti-Tb drug (RIF) was prepared according to manufacturer’s instructions. Concentration range for each tested extracts or fractions were 15.6–1000 μg/mL; tested concentration range for positive control drug (RIF) was 0.25–16 μg/mL

ISSN: 2662-7671