Fig. 7

Effects of SC-E1 on the Nrf2 activation in RAW 264.7 macrophages. a Nuclear accumulation of Nrf2. Nrf2 was immunoblotted in the nuclear fractions of the cells treated with 300 μg/ml of SC-E1 for 0.5–6 h. Immunoblotting for Lamin B verified the equal loading of nuclear proteins. Each experiment conducted for 3 repeats per conditions. (Significant compared to the control, *p < 0.05 and ***p < 0.001.) b Immunofluorescence. RAW 264.7 cells were treated with 300 μg/ml of SC-E1 for 3 h. The staining of Nrf2 and DAPI was conducted as described in the method section. c Immunoblotting for HO-1. The HO-1 was immunoblotted in the RAW 264.7 cells that had been treated with 10–300 μg/ml of SC-E1 for 3 h. d Inhibitory effects of SC-E1 pretreatment and co-pretreatments with SC-E1 and SnPP (a HO-1 inhibitor) on the LPS-induced production of nitrite. RAW 264.7 cells were pretreated with SC-E1 (μg/ml) for 1 h in the presence or absence of SnPP (50 nM, 30 min), and then stimulated with LPS (1 μg/ml) for 24 h