Table 2 Gene sequences of primer pairs and annealing temperatures

GAPDH forward

TGC ACC ACC AAC TGC TTA GC

60 °C

GAPDH reverse

GGC ATG GAC TGT GGT CAT GAG

60 °C

COL2A1 forward

GTT ATC GAG TAC CGG TCA CAG AAG

65 °C

COL2A1 reverse

AGT ACT TGG GTC CTT TGG GTT TG

65 °C

ACAN forward

CAG CAC CAG CAT CCC AGA

65 °C

ACAN reverse

CAG CAG TTG ATT CTG ATT CAC G

65 °C

COL1A1 forward

TGA CCT CAA GAT GTG CCA CT

65 °C

COL1A1 reverse

ACC AGA CAT GCC TCT TGT CC

65 °C

SOX9 forward

AGA CCT TTG GGC TGC CTT AT

60 °C

SOX9 reverse

TAG CCT CCC TCA CTC CAA GA

60 °C