Fig. 9
From: In vitro induction of human embryonal carcinoma differentiation by a crude extract of Rhazya stricta
RT-PCR analysis of stem cell genes in NT2 cells at different time points (7, 14, and 21 days) after treatment with retinoic acid (RA). Representative agarose gel electrophoresis patterns of RT-PCR products showed reduced expression of Oct4, Sox2, Nanog, and Klf4. All gene expression data were normalized to β-actin as an internal control. Band intensity was measured and the values were expressed as the mean ± SD of two independent experiments. *P < 0.05, **P < 0.01 compared to vehicle control (VC; 0.001% DMSO)