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Fig. 4 | BMC Complementary and Alternative Medicine

Fig. 4

From: 6-shogaol, a neuroactive compound of ginger (jahe gajah) induced neuritogenic activity via NGF responsive pathways in PC-12 cells

Fig. 4

Neuritogenic effect of 6-shogaol in PC-12 cells. a Percentage of neurite-bearing cells after 48 h of incubation with NGF (50 ng/ml) (positive control) or 6-shogaol (5 to 10,000 ng/ml). Cells in complete F-12 K medium served as a negative control. Data were expressed as means ± SD from three independent experiments carried out in triplicates. Means with different alphabets show significant differences (p < 0.05). Phase contrast photomicrographs of PC-12 cells: (b) in medium only; (c) treated with 50 ng/ml of NGF; (d) treated with 500 ng/ml of 6-shogaol. Scale bar = 20 μm. Immunocytofluorescence staining of neurofilaments of PC-12 cells: (e) in medium only; (f) treated with 50 ng/ml of NGF; (g) treated with 500 ng/ml of 6-shogaol. Scale bar = 50 μm. Arrows indicate neurite extensions

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