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Table 6 Anthelmintic activity of aerial part of Isodon rugosus against Pheretima posthuma and Ascaridia galli

From: Demonstration of biological activities of extracts from Isodon rugosus Wall. Ex Benth: Separation and identification of bioactive phytoconstituents by GC-MS analysis in the ethyl acetate extract

Samples

Concentration (mg/ml)

Pheretima posthuma

Ascaridia galli

Paralysis time in Minutes (mean ± SD)

Death time in Minutes (mean ± SD)

Paralysis time in Minutes (mean ± SD)

Death time in Minutes (mean ± SD)

Ir.Cr

10

24.33 ± 1.15***

67.00 ± 2.00***

20.25 ± 0.57***

69.39 ± 0.60***

20

18.67 ± 1.53**

60.00 ± 2.00***

17.01 ± 0.46***

61.18 ± 0.67***

40

11.33 ± 1.56ns

40.67 ± 1.53***

13.48 ± 0.52***

53.19 ± 0.36***

Ir.Hex

10

23.33 ± 2.08***

67.33 ± 2.08***

28.89 ± 0.52***

87.34 ± 0.78***

20

17.00 ± 1.00*

62.33 ± 1.53***

17.32 ± 0.96***

71.36 ± 0.56***

40

11.67 ± 1.53ns

49.00 ± 1.00***

3.43 ± 0.67**

56.63 ± 0.38***

Ir.Cf

10

14.00 ± 1.00ns

50.00 ± 1.00ns

14.53 ± 0.67ns

53.01 ± 0.44***

20

10.00 ± 2.00ns

37.67 ± 1.53ns

11.95 ± 0.34ns

41.77 ± 0.40ns

40

7.33 ± 1.53ns

30.67 ± 2.52ns

9.85 ± 0.50ns

34.39 ± 0.55ns

Ir.EtAc

10

18.00 ± 2.00ns

52.00 ± 2.00ns

18.13 ± 0.49***

58.37 ± 1.16***

20

13.00 ± 2.00ns

45.00 ± 2.00*

13.63 ± 0.40**

49.31 ± 0.82***

40

10.00 ± 2.00ns

38.67 ± 1.53**

10.29 ± 0.56*

42.25 ± 0.89***

Ir.Aq

10

21.00 ± 2.00**

61.00 ± 2.00***

19.66 ± 0.88***

68.54 ± 0.81***

20

19.00 ± 2.00**

56.00 ± 2.00***

15.4 ± 0.61***

57.15 ± 0.85***

40

15.00 ± 2.00**

47.67 ± 1.53***

13.40 ± 0.69***

52.85 ± 0.50***

Ir.Sp

10

13.33 ± 1.53ns

44.00 ± 2.00ns

8.40 ± 0.9***

43.00 ± 1.00**

20

9.00 ± 2.00ns

33.67 ± 3.05ns

8.33 ± 0.58ns

32.79 ± 0.54***

40

5.67 ± 1.53ns

27.67 ± 1.53ns

4.33 ± 0.58ns

29.22 ± 0.61**

Albendazole

10

12.33 ± 2.08

48.67 ± 1.53

13.10 ± 1.85

47.40 ± 1.50

20

10.33 ± 2.57

37.33 ± 1.53

9.80 ± 1.60

40.20 ± 2.25

40

7.00 ± 2.00

28.67 ± 1.53

7.10 ± 1.01

33.60 ± 1.76

Negative control

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  1. Values significantly different (*: P < 0.05, ** P < 0.01 and *** P < 0.001) as compared to positive control drug. ns: Non significantly different in comparion to control group
  2. Keys:
  3. Ir.Cr Crude methanolic extract, Ir.Hex n-hexane fraction, Ir.Cf Chloroform fraction, Ir.EtAc Ethyl acetate fraction, Ir.Aq Aqueous fraction, Ir.Sp Saponins.