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Fig. 2 | BMC Complementary and Alternative Medicine

Fig. 2

From: Wogonin suppresses stem cell-like traits of CD133 positive osteosarcoma cell via inhibiting matrix metallopeptidase-9 expression

Fig. 2

Effect of Wogonin on the migration ability of CD133+ CAL72 CSC. a Wound healing assay showed that the rate of healing decreased after Wogonin treatment (0, 10, 20, 40, and 80 μM). b The percentage of healing area was constrained by Wogonin treatment (0, 10, 20, 40, and 80 μM), as measured by ImageJ software at 24 h post treatment. c Migration of CD133+ CAL72 cells was inhibited by Wogonin treatment (0, 10, 20, 40, and 80 μM), as visualized by staining with 0.5% crystal violet. d The number of migrating cells was evaluated in five random fields in each trans-well insert exposed to varying concentrations of Wogonin (0, 10, 20, 40, and 80 μM). e OD573nm measurement after destaining with methanol and acetic acid. All experiments were performed in triplicates. Data are represented as mean ± SEM (n = 3). P < 0.05, P < 0.01, *** P < 0.001, **** P < 0.0001

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