Fig. 1

Wogonin induces apoptosis in CD133⁺ CAL72 CSC. a Western blotting was performed on 24 h-treated extract of CSC in the presence of varying concentrations of Wogonin (0, 10, 20, 40, 80 μM). b Percentage of annexin-V-positive cells after Wogonin treatment. c Percentage of apoptotic cells as analysed by flowcytometry. d The morphology of CSC was altered at 24 h post treatment; the changes in nuclear morphology were observed by Hoechst 33,342 staining after treating with the indicated Wogonin concentration (0, 10, 20, 40, 80 μM). All experiments were performed in duplicates. Data are represented as mean ± SEM (n = 3). ^∗ P < 0.05, ^∗∗ P < 0.01, ^**** P < 0.0001