Fig. 11

Immunohistochemical images of the spleen section, from mice treated with TME at the rate of 1500 mg/kg, 8 hourly intervals for three days consecutively. In the white pulp, PALS region of the spleen surrounding central artery (black arrows) appear darker as it is predominantly occupied by small lymphocyte in (a). In (b and c), detection of ssDNA in apoptotic cells, a marker for apoptosis is shown as a brown reaction due to DAB peroxidase reaction (dotted line arrows). The reaction is severe in the red pulp and areas on the marginal zone of the white pulp, some of the reactions are also seen in the PALS and the lymphoid follicles. Image D is from mice treated with PBSG at 0.1 ml/ 10 g as the extract at 8 hourly for three days consecutive for comparison. Magnifications, (a) 100 x; (b, c and d) 400 x