Fig. 7
Annexin-V & Dead Cell (7-AAD) flow cytometry analysis. Apoptotic effect of MP-HX and MP-EA on HCT116, HCC1937, HepG2 and MDA-MB-231 cell lines. (a) Apoptosis profile plot. Each plot is a representative figure of the three replicates of each determination. (b) Bar charts depicting percentage of live, dead and apoptotic cells for the treatments on the corresponding cell lines. Values are mean ± SD (n = 3). The values with different letters (a-d) are significantly different, p < 0.05. The concentration of the extracts used in each treatment was at ~IC50 value for the corresponding cell lines indicated. T24, 24 h treatment; T48, 48 h treatment; VC, vehicle control. L, live cells; EA, early apoptotic cells, LA, late apoptotic cells; D, dead cells