Fig. 4

Effects of ECAP on RANKL-induced NF-κB and NFATc1 activation. a RAW 264.7 cells were pre-incubated for 1 h with indicated concentrations of ECAP and then activated for 1 h with RANKL (50 ng/ml). Cytoplasm fractions were obtained for the detection of phospho-IκBα, phospho-p65 and β-actin levels. Nuclear fractions were obtained for the detection of p65, p50 and PCNA levels. b RAW 264.7 cells were pre-incubated for 1 h with indicated concentrations of ECAP and then activated for 24 h with RANKL (50 ng/ml). The cell lysates were obtained for the detection of NFATc1 levels. Each value represents the mean ± SD. (n = 3). ## P < 0.01 compared with control; *P < 0.05, **P < 0.01 compared with RANKL alone