Fig. 3
Effects of ECAP on mRNA and protein expression of steoclastogenesis-related genes. a RAW 264.7 cells were cultured in the presence of RANKL (50 ng/ml) with the vehicle (H2O) or ECAP. After 24 h, total RNA was then isolated using TRIzol reagent, and mRNA expression levels were evaluated by RT-PCR. Glyceraldehyde- 3-phosphate dehydrogenase (GAPDH) was used as the internal control. b RAW 264.7 cells were cultured in the presence of RANKL (50 ng/ml) with the vehicle (H2O) or ECAP. After 24 h, the culture medium was collected and analyzed by gelatin zymography. Each value represents the mean ± SD. (n = 3). ## P < 0.01 compared with control; *P < 0.05, **P < 0.01 compared with RANKL alone