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Fig. 2 | BMC Complementary and Alternative Medicine

Fig. 2

From: (−)-Epicatechin 3-O-β-d-allopyranoside prevent ovariectomy-induced bone loss in mice by suppressing RANKL-induced NF-κB and NFATc-1 signaling pathways

Fig. 2

Effects of ECAP on RANKL-induced osteoclastogenesis activity. a RAW 264.7 cells were cultured for 5 days in the presence of RANKL (50 ng/ml) with the vehicle (H2O) or ECAP. Multinucleated osteoclasts were visualized by TRAP staining. TRAP-positive cells were photographed under a light microscope. Multinucleated TRAP-positive cells with ≥3 nuclei were defined as osteoclasts. b RAW 264.7 cells were cultured on Osteoclast Activity Substrate plates in the presence of RANKL (50 ng/ml) with the vehicle (H2O) or ECAP for 7 days. After a 7-day culture, adherent cells were removed and photographed under a light microscope. Each value represents the mean ± SD. (n = 3). **P < 0.01 compared with RANKL alone

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