Fig. 4
From: HVC1 ameliorates hyperlipidemia and inflammation in LDLR−/− mice
HVC1 regulation of cholesterol metabolism and lipid synthesis in LDLR−/− mice. Total RNA was subjected to real-time PCR as described in the Methods section. Cholesterol and lipid metabolism-related genes mRNA levels were analyzed by real-time PCR analysis. (a) SREBP-2, (b) HMGCR, (c) LPL, (d) apoB, and (e) LXR. Protein levels of (f) SREBP-2, (g) HMGCR, (H) p-AMPK, and AMPK in liver tissues were analyzed by western blot. Proteins were determined by western blot assay using specific antibody. β-actin was used as a loading control. Values represent mean ± S.D. of three independent experiments (significant as compared to HCD, * p < 0.05, ** p < 0.01, *** p < 0.001, significant as compared to ND, ### p < 0.001)