Fig. 8

DOE inhibits the LPS-triggered release of HMGB1 by suppressing phosphorylation of JNK via NO in RAW 264.7 cells. a Cells were treated with LPS for the indicated amounts of time. b Cells were stimulated with vehicle (DMSO) or LPS in the presence or absence of DOE for 30 min. Aliquots of whole-cell lysates were immunoblotted with activation-specific antibodies, and parallel immunoblots were analyzed for total kinase levels. c and d Cells were pretreated with SP600125 for 1 h and then exposed to vehicle (DMSO) or LPS with or without DOE. Following incubation for 24 h, aliquots of conditioned media or whole-cell lysates were subjected to immunoblot or nitrite analysis to detect HMGB1 (c) or iNOS and nitrite (d), respectively. The blots are representative of three independent experiments. The results are plotted as the means ± S.E. (n = 5). ^*, p < 0.01 vs untreated group; ^#, p < 0.01 vs LPS-treated group