Fig. 6

AAE induced apoptosis through regulated mitochondria signaling pathways in HCT116 Xenograft model. a Tumor size and body weight of HCT116 Xenograft model mouse. AAE direct inject to tumor and tumor size were redialed each 2 day and tumor image. b Histological experiments of tumor tissue. H&E staining, TUNEL assay and Immunohistochemical (IHC) assay were were performed at 21 day. The arrow and around indicate that specific proteins were activated by AAE treatment. c The expression of PTEN, PDK1, Akt (Thr308 and Ser473), MDM2, p53, PUMA,Bcl-2, pro-caspase3 and the activation of Bax, Bak and cleaved PARP were analyzed by western blot analysis