Fig. 5

AOF extract effects on Schwann cell proliferation was IGF-1signaling dependent. RSC96 cells were pretreated with IGF1R inhibitor (a) or PI3K inhibitor (b) for 1 h, and then treated with 100 μg/ml AOF extract for 24 h. Western blot analysis showed that AOF-induced the up-regulation of p-PI3K, p-Akt and p-Bad which was markedly suppressed when treated with either AG1024 (IGF1R inhibitor; Promega, Madison, WI, USA) or LY294002 (PI3K inhibitor; Promega, Madison, WI, USA) inhibitor.α-tubulin was used as a load control. After incubation with AOF extract, migration assay was performed using Boyden chambers treated with IGF1R inhibitor and PI3K inhibitor, respectively (c and d). The number of migrated cells was much more in the cells treated with AOF extract, but the migration effect was blocked when treated with IGF1R inhibitor and PI3K inhibitor