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Table 1 Protocol for MGIT-DST of MDR-Mtb with Exo-MS alone and in combination with INH and RIF

From: Preliminary evaluation of anti-tuberculosis potential of siderophores against drug-resistant Mycobacterium tuberculosis by mycobacteria growth indicator tube-drug sensitivity test

No

Drug

Initial Concentration of the drug preparation

Volume added to MGIT for Test

Final Concentration in MGIT tube except GC

No. of Middle Brook tubes

GC

MDR

1

INH

8.3 μg/mL

100 μL

0.1 μg/mL

1

1

2

RIF

83 μg/mL

100 μL

1.0 μg/mL

 

1

3

Exo-MS + INH

19 mg/mL + 8.3 μg/mL

50 μL + 100 μL

0.125 mg/mL and 0.1 μg/mL

 

1

4

Exo-MS + INH

19 mg/mL + 8.3 μg/mL

100 μL + 100 μL

0.25 mg/mL and 0.1 μg/mL

 

1

5

Exo-MS + INH

19 mg/mL + 8.3 μg/mL

200 μL + 100 μL

0.5 mg/mL and 0.1 μg/mL

1

1

6

Exo-MS + RIF

19 mg/mL + 83 μg/mL

50 μL + 100 μL

0.125 mg/mL and 1.0 μg/mL

 

1

7

Exo-MS + RIF

19 mg/mL + 83 μg/mL

100 μL + 100 μL

0.25 mg and 1.0 μg/mL

 

1

8

Exo-MS + RIF

19 mg/mL + 83 μg/mL

200 μL + 100 μL

0.5 mg and 1.0 μg/mL

 

1

9

Exo-MS

19 mg/mL

50 μL

0.125 mg/mL

1

1

10

Exo-MS

19 mg/mL

100 μL

0.25 mg/mL

 

1

11

Exo-MS

19 mg/mL

200 μL

0.5 mg/mL

 

1

12

Exo-MS+ FeCl3

19 mg/mL + 80 mg/mL

200 μL + 50 μL

0.5 mg/mL and 0.5 mg/mL

 

1