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Table 4 Antibacterial activities of leaf and stem extracts of R. punjabensisin terms of zone of inhibition (ZOI) and their MICs

From: Appraisal of phytochemical and in vitro biological attributes of an unexplored folklore: Rhus Punjabensis Stewart

Extracts

Zone of inhibition (mm) at 100 μg/disc and MIC (μg/mL)

P. aeruginosa

MIC

S. typhimurium

MIC

M. luteus

MIC

B. bronchiseptica

MIC

S. aureus

MIC

Leaf

 n-hex

--

--

--

--

--

--

13 ± 1.24***

33.3c

12 ± 1.17*

33.3c

 CHCl3

--

--

--

--

--

--

08 ± 0.49

--

11 ± 1.16

100

 Acet

10 ± 1.15

100

13 ± 1.29*

33.3

18 ± 1.85***

11.11ab

14 ± 1.23**

33.3c

08 ± 0.66

--

 EtOAc

10 ± 1.14

100

22 ± 2.25***

1.11a

14 ± 1.12

11.11ab

11 ± 1.19

100

12 ± 1.26

100

 EtOH

06 ± 0.56

--

10 ± 1.19

100

13 ± 1.56

33.3c

12 ± 1.11*

100

11 ± 1.18

100

 MeOH

14 ± 1.66*

33.3ab

--

--

--

 

06 ± 0.25

--

18 ± 19.6

11.11ab

 EtOH+ CHCl3

10 ± 1.11

100

10 ± 1.18

100

18 ± 1.65***

11.11ab

16 ± 1.59**

33.3c

08 ± 0.77

--

 MeOH+ CHCl3

--

--

13 ± 1.55

33.3c

10 ± 1.13

33.3c

09 ± 0.98

--

09 ± 0.85

--

 EtOH + EtOAc

12 ± 1.55

33.3ab

18 ± 2.98***

11.3ab

15 ± 1.44**

11.11ab

10 ± 1.12

100

13 ± 1.26**

33.3

 MeOH + EtOAc

13 ± 1.65*

33.3ab

15 ± 1.12**

11.11cb

15 ± 1.73**

11.11ab

10 ± 1.12

100

08 ± 0.66

--

 Acet + EtOAc

15 ± 1.84**

11.11a

14 ± 1.51*

11.11cb

20 ± 2.93***

1.11a

11 ± 1.11

100

13 ± 1.56

33.3c

Stem

 n-hex

--

--

13 ± 1.37

100

10 ± 1.17

--

12 ± 1.24

100

14 ± 1.97**

33.3c

 CHCl3

05 ± 0.78

--

10 ± 1.14

100

--

--

11 ± 1.14

--

06 ± 0.57

--

 Acet

11 ± 1.27

100

15 ± 1.58**

33.3

11 ± 1.15

100

13 ± 1.67**

33.3c

10 ± 1.16

--

 EtOAc

11 ± 1.26

100

11 ± 1.71

100

14 ± 1.29**

33.3c

13 ± 1.68

--

10 ± 1.17

--

 EtOH

10 ± 1.17

100

10 ± 1.17

100

10 ± 1.15

--

13 ± 1.74

--

15 ± 1.53**

33.3c

 MeOH

--

--

07 ± 0.88

--

--

--

10 ± 1.14

--

12 ± 1.25

--

 EtOH+ CHCl3

--

--

07 ± 0.79

--

12 ± 1.19

100

12 ± 1.12

--

10 ± 1.17

--

 MeOH+ CHCl3

16 ± 1.97

33.3ab

07 ± 0.89

--

16 ± 1.67**

11.1ab

08 ± 0.41

--

11 ± 1.27

--

 EtOH + EtOAc

13 ± 1.10**

33.3ab

12 ± 1.10

100

11 ± 1.27

 

15.5 ± 1.97**

11.11ab

11 ± 1.21

--

 MeOH + EtOAc

08 ± 0.98

 

10 ± 1.55

 

12 ± 1.37

100

09 ± 0.77

--

08 ± 0.89

--

 Acet + EtOAc

11 ± 1.81

100

12 ± 1.73

100

11 ± 1.21

--

10 ± 1.18

--

08 ± 0.88

--

 Cefixime

26 ± 2.98

1.1

25 ± 2.93

1.1

24 ± 2.71

1.1

24 ± 2.92

1.1

23.5 ± 2.71

1.1

 Roxithromycin

26.5 ± 2.95

0.1

25 ± 2.53

0.1

26.5 ± 2.84

0.1

26 ± 2.85

0.1

25 ± 2.32

0.1

  1. Values (mean ± SD) are average of three samples of each plant extract, analyzed individually in triplicate (n = 1x3). Means difference is highly significant*** slightly significant**, and significant* at p < 0.05. The MIC values within the column are statistically analyzed using LSD test at p <0.05. The same alphabates represent non-significant difference -- = No activity in antibacterial assay or not active (zone ˂ 10 mm) for MIC determination