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Fig. 3 | BMC Complementary and Alternative Medicine

Fig. 3

From: Effect of microbial siderophores on mammalian non-malignant and malignant cell lines

Fig. 3

HPLC of pure ferri-mycobactin S. MBS at 4 mg/mL concentration was analyzed by injecting 10 μL volume in Waters Xterra MS MS HPLC system. The column used was C18, 5 μm, 4.6 × 100 mm at the flow-rate of 1.5 mL per min. Solvent system was methanol: water (80:20, v/v) for 30 min followed by 100% methanol for 15 min. (a) at 450 nm; (b) 220 nm. Seven peaks of different intensities were observed depending upon the acyl chain length that varies from 9 to 19 carbons; the shortest chain being eluted out first and the longest last

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