Fig. 4

For whole cell currents in voltage clamped HL-1 cardiac cells, K⁺-currents were blocked by intracellular and extracellular Cs²⁺, and Na⁺- and T-currents inactivated by a -40 mV holding potential, then I_{Ca, L} activated by stepping to +10 mV (voltage profile: see inset). a Typical trace showing inhibition of I_{Ca, L} by 100 μg/ml B. monnieri in the same cell. b Inhibition by 100 μg/ml B. monnier of I_{Ca, L} expressed as percentage of mean compared to average of control I_{Ca, L} from the same cell. Data are means ± SEM (n = 4 cells from 3 cultures) and *** p < 0.001 comparing B. monnieri vs control, paired t-test