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Table 1 Combination of 4-OHT with BD regulates expression of cancer progression related genes

From: BreastDefend enhances effect of tamoxifen in estrogen receptor-positive human breast cancer in vitro and in vivo

Gene

Description

4-OHT (RQ)

BD (RQ)

Combination (RQ)

BRAF

serine/threonine-protein kinase B-Raf

2.91 ± 0.55*

1.85 ± 0.10

5.27 ± 0.82*

PTK2B

protein tyrosine kinase 2 beta

2.76 ± 0.62

2.25 ± 1.33

4.51 ± 1.41*

CDKN1A

cyclin-dependent kinase inhibitor 1A (p21)

1.06 ± 0.14

3.26 ± 0.28*

4.06 ± 0.22*

NFKBIA

NF-kappa-B inhibitor

1.86 ± 0.50

2.35 ± 0.33*

3.42 ± 0.16*

FYN

FYN oncogene related to SRC, FGR, YES

1.81 ± 0.34

2.36 ± 0.81

3.16 ± 0.81*

CASP9

caspase 9

2.04 ± 0.33

1.67 ± 0.16

2.67 ± 0.67*

BCL2

B-cell CLL/lymphoma 2

0.13 ± 0.10*

0.29 ± 0.23*

0.08 ± 0.05*

CCND2

cyclin D2

0.39 ± 0.08*

0.12 ± 0.10*

0.10 ± 0.10*

FN1

fibronectin 1

0.41 ± 0.04*

0.30 ± 0.09*

0.19 ± 0.11*

ITGA2B

integrin, alpha 2b

0.62 ± 0.12*

0.58 ± 0.20*

0.41 ± 0.12*

  1. DNA-microarray analysis was performed on TaqMan® Array Human Molecular Mechanisms of Cancer as described in Materials and Methods. MCF-7 cells were stripped of steroids for 3 days before seeding by culturing in steroid-free medium. After 24 h seeded into 6-well plates, cells were treated with E2 (10 nM) plus 4-OHT (1 μM), BD (10 μg/ml) or a combination of both for 6 days in steroid-free medium. Data are the means ± SD of three independent experiments. Analysis of the RQ gene expression data was performed using the 2-∆∆CT method. Statistical analysis by ANOVA *P < 0.05