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Table 1 Combination of 4-OHT with BD regulates expression of cancer progression related genes

From: BreastDefend enhances effect of tamoxifen in estrogen receptor-positive human breast cancer in vitro and in vivo

Gene Description 4-OHT (RQ) BD (RQ) Combination (RQ)
BRAF serine/threonine-protein kinase B-Raf 2.91 ± 0.55* 1.85 ± 0.10 5.27 ± 0.82*
PTK2B protein tyrosine kinase 2 beta 2.76 ± 0.62 2.25 ± 1.33 4.51 ± 1.41*
CDKN1A cyclin-dependent kinase inhibitor 1A (p21) 1.06 ± 0.14 3.26 ± 0.28* 4.06 ± 0.22*
NFKBIA NF-kappa-B inhibitor 1.86 ± 0.50 2.35 ± 0.33* 3.42 ± 0.16*
FYN FYN oncogene related to SRC, FGR, YES 1.81 ± 0.34 2.36 ± 0.81 3.16 ± 0.81*
CASP9 caspase 9 2.04 ± 0.33 1.67 ± 0.16 2.67 ± 0.67*
BCL2 B-cell CLL/lymphoma 2 0.13 ± 0.10* 0.29 ± 0.23* 0.08 ± 0.05*
CCND2 cyclin D2 0.39 ± 0.08* 0.12 ± 0.10* 0.10 ± 0.10*
FN1 fibronectin 1 0.41 ± 0.04* 0.30 ± 0.09* 0.19 ± 0.11*
ITGA2B integrin, alpha 2b 0.62 ± 0.12* 0.58 ± 0.20* 0.41 ± 0.12*
  1. DNA-microarray analysis was performed on TaqMan® Array Human Molecular Mechanisms of Cancer as described in Materials and Methods. MCF-7 cells were stripped of steroids for 3 days before seeding by culturing in steroid-free medium. After 24 h seeded into 6-well plates, cells were treated with E2 (10 nM) plus 4-OHT (1 μM), BD (10 μg/ml) or a combination of both for 6 days in steroid-free medium. Data are the means ± SD of three independent experiments. Analysis of the RQ gene expression data was performed using the 2-∆∆CT method. Statistical analysis by ANOVA *P < 0.05