Fig. 3

Combination of 4-OHT with BD regulates expression of apoptosis related proteins. a MCF-7 cells were treated for 6 days as described in Fig. 1. Whole protein extracts isolated from cells were prepared and western blot analysis with anti-Raf-B, anti-p21, anti-Bcl-2, anti-Fibronectin and anti-β-actin antibodies were performed as described in Materials and Methods. β-actin was used as loading control and representative blots from three experiments were shown. b Quantitative data composed of all the experiments in MCF-7 cells with statistical analysis were on the right of the representative blot image. Statistical analysis by ANOVA and Holm-Sidak. Different letters above bars indicate significant differences P < 0.05. The graphical data represent mean +/− SD