Fig. 7From: Promotion of HepG2 cell apoptosis by flower of Allium atroviolaceum and the mechanism of actionReal-time quantitative PCR analysis illustrates the gene expression in HepG2 cells. The relative quantification of the target gene, (a) Bcl-2, (b) Cdk1 and (c) p53, by the delta–delta–Ct method was done using the Qiagen software after treatment with IC25 = 42, IC50 = 26.67 and IC75 = 11.67 of FAA for 24 h. A sample without cDNA template (ntc) was used as the negative control. Values are means ± SD of three independent experimentsBack to article page