Effects of phenolic constituents of daylily flowers on corticosterone- and glutamate-treated PC12 cells

Tian, Huan; Yang, Fei-Fei; Liu, Chun-Yu; Liu, Xin-Min; Pan, Rui-Le; Chang, Qi; Zhang, Ze-Sheng; Liao, Yong-Hong

doi:10.1186/s12906-017-1582-x

BMC Complementary Medicine and Therapies

Table. 1 Effects of Hemerocallis citrina fractions (HF) on the extracellular levels (ng/mg proteins) of dopamine (DA), 5-hydroxy tryptamine (5-HT), γ-aminobutyric acid (GABA), noradrenaline (NE) and acetylcholine (ACh) in corticosterone (Cort) or glutamate (Glu)-treated PC12 cells (mean ± SD, n = 5)

From: Effects of phenolic constituents of daylily flowers on corticosterone- and glutamate-treated PC12 cells

	Analyte	EtOH % extract	Control	Cort + Vehicle	Cort + HF (1.25 mg/mL)	Cort + HF (2.5 mg/mL)	Cort + HF (5 mg/mL)
Cort	DA	10%	117.18 ± 14.18	67.40 ± 11.56^##	98.01 ± 12.04	101.65 ± 24.17**	100.06 ± 24.60**
		30%	157.50 ± 34.50	81.48 ± 49.68^##	136.33 ± 42.53	127.50 ± 7.94**	136.12 ± 18.09**
		50%	134.18 ± 39.12	87.23 ± 13.26^##	103.78 ± 14.86	113.98 ± 25.40	117.15 ± 28.17
		10%	0.88 ± 0.04	0.60 ± 0.03^##	0.78 ± 0.21*	0.91 ± 0.03**	0.91 ± 0.08**
	NE	30%	1.48 ± 0.07	1.18 ± 0.16^#	1.54 ± 0.10*	1.58 ± 0.35*	1.68 ± 0.06**
		50%	1.34 ± 0.17	0.83 ± 0.20^##	1.42 ± 0.26**	1.42 ± 0.27**	1.49 ± 0.17**
		10%	15.53 ± 6.20	7.88 ± 4.26^##	11.89 ± 3.10	12.88 ± 2.65	13.76 ± 2.45
treated	ACh	30%	20.60 ± 0.70	13.57 ± 3.05^##	19.23 ± 1.85*	20.02 ± 5.05*	20.42 ± 5.25*
cells	GABA	50%	20.88 ± 4.56	8.35 ± 2.41^##	18.46 ± 0.67**	19.38 ± 2.97**	21.07 ± 3.39**
		10%	97.59 ± 56.99	39.52 ± 14.56^##	41.62 ± 16.01	57.18 ± 13.74	58.78 ± 12.29
		30%	153.03 ± 20.91	102.35 ± 12.87^##	120.32 ± 15.55	125.12 ± 37.04	123.93 ± 33.42
	5-HT	50%	114.70 ± 38.33	55.43 ± 19.63^##	112.06 ± 16.21*	113.40 ± 40.61*	111.75 ± 4.24*
		10%	159.67 ± 20.00	107.76 ± 7.93^##	119.09 ± 14.71	129.33 ± 17.66	121.22 ± 38.25
		30%	195.50 ± 47.78	111.15 ± 31.92^##	170.26 ± 38.25	204.25 ± 32.39**	205.86 ± 48.56**
	DA	50%	150.03 ± 8.61	93.04 ± 9.03^##	138.74 ± 31.51*	139.38 ± 31.00*	152.20 ± 24.80**
		10%	29.97 ± 1.06	11.54 ± 2.88^##	19.68 ± 8.22	24.16 ± 9.74*	26.74 ± 5.63**
		30%	49.40 ± 11.72	18.66 ± 9.04^##	24.11 ± 13.55	28.6 ± 3.65	29.62 ± 7.75
	NE	50%	50.90 ± 23.14	19.61 ± 8.07^##	28.40 ± 9.75	29.95 ± 10.18	29.64 ± 7.41
		10%	1.94 ± 0.32	1.53 ± 0.12^#	2.00 ± 0.23	2.00 ± 0.22*	1.99 ± 0.37*
		30%	2.24 ± 0.10	1.58 ± 0.03^##	1.91 ± 0.22**	1.98 ± 0.19**	1.99 ± 0.23**
		50%	1.96 ± 0.14	1.50 ± 0.14^##	1.77 ± 0.19	1.88 ± 0.18*	1.83 ± 0.19*
Glu		10%	36.51 ± 1.49	25.97 ± 2.68^##	27.23 ± 4.63	28.81 ± 6.76	30.95 ± 2.94
treated	ACh	30%	47.60 ± 19.10	28.45 ± 1.47^#	36.79 ± 5.23	42.43 ± 3.50*	43.65 ± 3.90*
cells		50%	70.42 ± 13.11	32.36 ± 3.43^##	51.48 ± 3.93*	53.71 ± 12.87**	57.03 ± 18.05**
		10%	133.73 ± 11.64	51.84 ± 6.36^##	88.69 ± 12.20**	101.46 ± 19.89**	105.08 ± 16.04**
	GABA	30%	163.25 ± 6.36	74.51 ± 27.86^##	128.30 ± 44.41*	133.05 ± 20.81*	133.88 ± 28.40*
		50%	198.50 ± 16.74	90.44 ± 25.83^##	151.50 ± 18.64**	154.75 ± 17.21**	150.94 ± 31.12**
		10%	224.49 ± 24.98	152.40 ± 18.27^##	177.35 ± 22.54	175.73 ± 36.75	183.31 ± 28.40
	5-HT	30%	211.75 ± 22.14	166.51 ± 24.52^#	172.98 ± 36.20	205.05 ± 19.46*	207.58 ± 24.62*
	5-HT	50%	173.50 ± 15.63	107.54 ± 32.38^##	152.60 ± 20.64**	156.50 ± 23.87**	154.02 ± 9.52**

^## P < 0.01 as compared with control group; *P < 0.05 and **P < 0.01 as compared with the corticosterone/glutamate group

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