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Fig. 7 | BMC Complementary and Alternative Medicine

Fig. 7

From: Cutaneous neurogenic inflammation in the sensitized acupoints induced by gastric mucosal injury in rats

Fig. 7

Distribution of 5-HT or HA co-expressed with tryptase (+) mast cells in EB extravasated dots. a, b. Representative triple-labeling immnuofluoresence staining images of 5-HT or HA, tryptase and DAPI. Triple-labeling immunofluorescent staining of mast cells was performed with either anti-5-HT (A, a, a1 in green) or anti-HA (B, b, b1 in green) and anti-tryptase (A, a, a2, B, b, b2, in red). The nuclei were labeled by fluorescent DAPI (in blue). Triple-labeled mast cells in the dermis co-expressed 5-HT and tryptase or HA and tryptase (white arrow). The mast cells aggregate (B, b) and degranulate (a2, b2 arrow) with 5-HT (+) or HA (+) granules (a1, b1). C, D. The number of aggregated and degranulated mast cells were markedly increased in EB dots than in beside EB dots and the control (*** P < 0.001 vs BEB and Control)

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