Fig 4

Distribution of SP immune-positive fibers in extravasated dots, beside EB, and in saline control. a, b. Representative immnuofluoresence staining images of SP in each group. SP (+) fibers are expressed mainly in the dermis (white boxes). a The few SP (+) fibers in dermis of skin in saline-control (white box). The corresponding images below were enlargements of the boxes outlined in a and b, and showed the nerve fibers of SP (+) (white arrow). b Distribution of SP (+) fibers in EB extravasated dots (b2 and b3) and beside EB dots (b1). Note the box was the location of SP (+) nerve fibers. SP labelled nerve fibers in EB dots were distributed more densely under the dermis than they were around the EB dots and in the control. b2 and b3 displayed SP (+) fibers forming a nerve plexus in the dermis. c, d. Sum fluorescence intensity and sum length (μm) of SP (+) fibers per section were expressed significantly more in EB than in the control and BEB (** P < 0.01 vs Control; # P < 0.05, vs BEB; ## P < 0.01, vs BEB)