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Fig. 4 | BMC Complementary and Alternative Medicine

Fig. 4

From: Plantamajoside from Plantago asiatica modulates human umbilical vein endothelial cell dysfunction by glyceraldehyde-induced AGEs via MAPK/NF-κB

Fig. 4

PM regulation of glycer-AGE-mediated pro-inflammatory cytokines in HUVECs with monocytes and THP-1. After the HUVECs and THP-1 cells were co-cultured, they were co-treated with PM (10 μM) and NAC (1000 μM) including glycer-AGE (100 μg/mL) for 6 h. After the treatments, the pro-inflammatory cytokines were measured using qRT-PCR. a mRNA was collected with TRIzol reagent and analyzed by 1.2–2% agarose gel with GAPDH as a control. The relative changes in mRNA bands were measured by Image J software. b qRT-PCR was analyzed by HiPi Real-timePCR 2X Master Mix SYBR green (Elpis Biotech, Seoul, Korea) using iQ5 thermal cycler (Bio-rad, CA, USA) with GAPDH as a control. Significance differences were analyzed with Duncan’s multiple range test as means ± SD and indicated by p < 0.05

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