Fig. 3
PM regulation of glycer-AGE-mediated monocyte and THP-1 adhesion to HUVECs. HUVECs were co-treated with PM (10 μM) and NAC (1000 μM) including glycer-AGEs (100 μg/ml) for 24 h, and then THP-1 cells were co-cultured on the HUVECs for 1 h in a dark CO2 incubator. Before the co-culture, the THP-1 cells were pre-labeled by BCECF-AM (100 μM) for 30 min. After the co-culture periods, the unattached monocytes were washed twice with PBS. a BCECF-AM-labeled monocytes were detected using confocal microscopy (100× magnification). b After the co-culture periods, cells lysis by 0.1% SDS in 50 mM Tris-buffer (pH 7.0) was detected using a fluorescence multi-plate reader in excitation 485 nm, emission 535 nm. The fluorescence was quantified and analyzed with Duncan’s multiple range test as means ± SD. Significant differences were indicated by p < 0.05