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Fig. 2 | BMC Complementary and Alternative Medicine

Fig. 2

From: Plantamajoside from Plantago asiatica modulates human umbilical vein endothelial cell dysfunction by glyceraldehyde-induced AGEs via MAPK/NF-κB

Fig. 2

PM regulation of receptor for AGEs (RAGE) and adhesion molecule expression in HUVECs treated with glycer-AGEs. HUVECs were co-treated with various concentrations of PM (10 μM) and NAC (1000 μM) including with glycer-AGEs (100 μg/mL) for 24 h, and then the cell lysates were obtained and analyzed with Western blotting or qRT-PCR. a Total cell lysates were obtained by RIPA that contained protease inhibitors and separated by 10% SDS-PAGE with GAPDH as a control. The relative changes in protein bands were measured by Image J software. b mRNA was collected with TRIzol reagent and analyzed by 1.2-2% agarose gel with GAPDH as a control. The relative changes in mRNA bands were measured by Image J software. c Quantitative real-time PCR (qRT-PCR) analyzed by HiPi Real-timePCR 2X Master Mix SYBR green (Elpis Biotech, Seoul, Korea) and analyzed using iQ5 thermal cycler (Bio-rad, CA, USA) with GAPDH as a control. Significance differences were analyzed with Duncan’s multiple range test as means ± SD at p < 0.05

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