Fig. 2From: ViscumTT induces apoptosis and alters IAP expression in osteosarcoma in vitro and has synergistic action when combined with different chemotherapeutic drugsViscumTT induces apoptosis synergistically and leads to loss of mitochondrial membrane potential (∆Ψm). The means ± SD of apoptotic cells are shown (a). Mistletoe lectin (ML) and oleanolic acid (OA) concentrations were used as a measure of viscum and TT. (p ≤ 0.05, n ≥ 3, *Fp > 1 = synergism). Apoptosis was confirmed by proteolytic processing of CASP3 and cleavage of PARP (b). β-actin was used as loading control. The means ± SD of loss of mitochondrial membrane potential (∆Ψm) (c) and carbonyl cyanide 3-chlorophenylhydrazone (CCCP was used as positive control (p ≤ 0.05, n ≥ 3, *Fp > 1 = synergism) are shown. Cytochrome c (d) release confirmed mitochondrial pathway activation. β-actin served as loading controlBack to article page