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Fig. 2 | BMC Complementary and Alternative Medicine

Fig. 2

From: Litchi seed extract inhibits epidermal growth factor receptor signaling and growth of Two Non-small cell lung carcinoma cells

Fig. 2

Cell-cycle arrest in NSCLC cells by LCSE. LCSE-treated cells were incubated at 37 °C for 24 h, fixed in 70% alcohol and stained with propidium iodide, then analyzed by flow cytometry, as described in Materials and Methods. The same LCSE-treated cells were lysed and the cell proteins separated by SDS-PAGE followed by immunoblotting to show proteins as indicated, with the beta-actin level as the loading control. Protein levels were quantified using Image Lab software (Bio-Rad) according to the density of each band on the immunoblotting image, normalized to the reference band (β-actin) and presented as the fold change of the untreated control. The cell-cycle distributions of LCSE-treated A549 and NCI-H661 cells are shown in (a), respectively, and the cell-cycle-associated proteins are shown in (b). The data reported are the percentages of total cells from the averages of three independent experiments and are expressed as means ± SD. *P < 0.05

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