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Fig. 3 | BMC Complementary and Alternative Medicine

Fig. 3

From: The Benzopyrone Biochanin-A as a reversible, competitive, and selective monoamine oxidase B inhibitor

Fig. 3

BIO-A and PCSEE selectively inhibited H2O2 produced by hMAO-B more than hMAO-A using a spectrophotometric assay without scavenging H2O2, (a) PCSEE inhibited both isozymes from producing H2O2, and inhibits hMAO-B selectively. b BIO-A inhibited hMAO-A and hMAO-B with a higher potency and hMAO-B selectivity (RSB) than PCSEE. c H2O2 scavenging activity by PCSEE and BIO-A compared to ascorbic acid. d Fluorescent TLC plate of PCSEE fingerprint and a BIO-A matching band (R f  ± SEM) visualized under UV waves. Data points were presented as the mean ± SEM, n = 3 (a, b), 4 (c), and 6 (d) IC50 ± SEM is interpolated from two sigmoidal curves averages. Significance of differences between hMAO-A and hMAO-B inhibition curves in (a) and (b) were determined using a two-way ANOVA followed by Sidak’s multiple comparisons test, and between control and treatments in (c) using one-way ANOVA followed by Dunnett’s multiple comparisons test; *p ≤ 0.05, ****p < 0.001

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