Fig. 7
EL treatment of lung cancer cells alters levels of FAK-Src signaling proteins. a-d A549 or e-h H460 cells were treated with 100 μM EL for 0, 1, 3, 6, 12, or 24 h. Western blotting was done to identify changes in total or phosphorylated levels of FAK, Src, paxillin, RhoA, and Rac/Cdc42 proteins. The blots represent one typical result from three independent experiments. Phosphorylated levels of proteins were normalized to their respective total protein levels. Alternatively, GAPDH was used as a loading control. Densitometry was performed using ImageJ software. p ≤ 0.05 was considered statistically significant when compared with untreated control