Fig. 2

EL impairs the in vitro migration potential of lung cancer cells independent of cell proliferation. a A549 and c H460 cells were grown to 90% confluency in cell culture dishes. A scratch/wound was made in each dish. The cells were then treated with 0, 10, 50, and 100 μM EL for 24 or 48 h. Images were taken at each time point for the respective control and treatment groups. The distance across the wound was measured for three replicate experiments for b A549 and d H460 cells and quantified as the % migration index. Ki-67 staining and quantification of e and f A549 and g and h H460 cells were performed to identify the % of Ki-67 positive cells near the wound. The data represent the average ± standard deviation % migration index for three fields per treatments for three independent experiments. p ≤ 0.05 was considered statistically significant when compared with untreated control