Table 3 MRM chromatogram by UHPLC/MIS/MS method for each subject from a normal assay sample
From: Triptolide Induces hepatotoxicity via inhibition of CYP450s in Rat liver microsomes
component | Corresponding CYP450s | parent ion | daughter ion | Fragmentor /V | Collision energy/V | polarity |
|---|---|---|---|---|---|---|
Caffeine(CAF) | CYP1A2 | 195.0 | 137.8 | 100 | 20 | ESI+ |
Tolbutamide(D860) | CYP2C9 | 269.1 | 169.8 | 110 | 13 | ESI- |
(S)-Mephenytoin(MT) | CYP2C19 | 219.0 | 134.0 | 100 | 17 | ESI+ |
Dextromethorphan(DM) | CYP2D6 | 272.0 | 214.9 | 155 | 25 | ESI+ |
Chlorzoxazone(CLZ) | CYP2E1 | 168.0 | 131.8 | 120 | 17 | ESI- |
Midazolam(MDZ) | CYP3A | 326.0 | 291.0 | 106 | 25 | ESI+ |
Paraxanthine | CYP1A2 | 181.0 | 123.9 | 120 | 21 | ESI+ |
4-Hydroxy Tolbutamide | CYP2C9 | 287.0 | 74.2 | 95 | 13 | ESI+ |
(+/−)-4-Hydroxy Mephenytoin | CYP2C19 | 235.0 | 150.0 | 90 | 17 | ESI+ |
Dextrorphan | CYP2D6 | 258.2 | 157.0 | 145. | 40 | ESI+ |
6-Hydroxy chlorzoxazone | CYP2E1 | 183.9 | 64.1 | 100 | 33 | ESI- |
1-Hydroxymidazolam | CYP3A | 342.0 | 324 | 162 | 21 | ESI+ |
Diazepam(DZP) | ISTD | 285.0 | 193.0 | 159 | 33 | ESI+ |