Table 2 Primers used in RT-PCR
From: Triptolide Induces hepatotoxicity via inhibition of CYP450s in Rat liver microsomes
Target gene | Sequence of primers | |
|---|---|---|
CYP3A1/3A23 | forward | TTTCCTTTGTCCTGCATTCC |
reverse | CATAGGTGGGAGGTGCCTTA | |
CYP3A2 | forward | CGTCGATTCCCTTAACAACC |
reverse | AATCCTTTGGGAACATGCAG | |
CYP3A9 | forward | ACAAAGACCCGCATTACTGG |
reverse | GCAAACCTCATGCCAATACA | |
CYP3A18 | forward | CCAATAAGGCACCTGTCACC |
reverse | AGGGTTCCGATGAAGAGGAT | |
CYP3A62 | forward | GATGTGGAGATTGTGGCTCA |
reverse | TCTGGAGATCAGGGTGTGTG | |
CYP2C9 | forward | CTGCTGCTGCTGAAACACGTG |
reverse | GGATGACAGCGTACTATCAC | |
β-actin | forward | TCACCCACACTGTGCCCATCTATGA |
reverse | CATCGGAACCGCTCATTGCCGATAG | |