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Fig. 8 | BMC Complementary and Alternative Medicine

Fig. 8

From: Triptolide Induces hepatotoxicity via inhibition of CYP450s in Rat liver microsomes

Fig. 8

Verification in mRNA and protein level. a Effect of triptolide on mRNA levels of CYP3A family and CYP2C9 in livers of rats. Liver lysates were made from livers of rats. b Protein lysates were made from microsomes of rats’ liver and subjected to Western blot analysis using the indicated antibodies. The GAPDH was used as an internal control. A representative blot from three rats in each group was shown. The density of the immunoreactive bands was analyzed as(c). d Immunohistochemical detection of CYP2C19 and CYP2E1 in livers section of rats was shown. The Immunoreactive cells were stereo logically quantified (e). Symbols and bars represent the mean ± SD, respectively; *p < 0.05, **p < 0.01 versus the control group

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