Fig. 7

Effects of TP on the CYP450s kinetic parameters. a-f The Michaelis–Menten data of each CYP450s. After orally treated with vehicle or 200, 400 or 600 μg/kg/day of triptolide for 28 days, the liver microsomes incubated with the cocktail probe (CAF/D860/MT/DM/CLZ/MDZ) in different concentration (diluted with half dilution method as listed in Table 1) and a NADPH-generating system at 37 °C. The concentration of the each metabolites were detected by UPLC-MS/MS at the incubation time of 1, 2, 4, 6, 10 min, the rate of metabolism were calculated from linear regression analysis. The rate of metabolism [nmol/(protein g × min)] versus the concentration of each substrates was fit to The Michaelis–Menten equation. Data represents the mean of three independent experiments in duplicate determinations. g-h The Km (g) and Vmax values (h) were calculated from non-linear regression analysis of experimental data according to the Michaelis–Menten models for the kinetics of each CYP450s with Prism 5.0 program. Results are expressed as mean ± SD of each group, respectively;*P < 0.05, ** P < 0.01 significantly different from the control group