Table 1 Half maximal inhibitory concentration (IC50) value of LFE and standards compounds for different free radical scavenging assays
S.No | Assay | Standard compound | Calculated IC50 Standard | Calculated IC50 LFE | 2-sample t test (p value) |
|---|---|---|---|---|---|
1 | DPPH | Ascorbic acid | 11.30 ± 1.23 | 3.23 ± 0.7 | 0.002 |
2 | Superoxide radical | Quercetin | 63.83 ± 2.5 | 65.57 ± 3.4 | 0.52 |
3 | Singlet Oxygen | Lipoic acid | 131.21 ± 8.3 | 162.72 ± 4.2 | 0.02 |
4 | Total antioxidant assay | Trolox | 3.26 ± 1.7 | 3.89 ± 0.5 | 0.6 |
5 | Hypochlorous scavenging activity | Ascorbic acid | 87.72 ± 4.9 | 124.03 ± 9.1 | 0.009 |
6 | Hydroxyl radical | Mannitol | 332.93 ± 3.5 | 124.75 ± 5.8 | 0.00001 |
7 | Nitric oxide | Curcumin | 109.60 ± 6.1 | 58.86 ± 7.5 | 0.0028 |
8 | Peroxynitrite radical | Gallic acid | 591.65 ± 13.9 | 500 ± 12.2 | 0.003 |
9 | Erythrocyte membrane stabilizing activity | Quercetin | 94.74 ± 5.5 | 152.48 ± 4.9 | 0.0008 |
10 | Fe chelation | EDTA | 25.37 ± 3.5 | 118.771 ± 12.4 | 0.0062 |