Fig. 4
From: SH003 induces apoptosis of DU145 prostate cancer cells by inhibiting ERK-involved pathway
SH003 induces apoptotic cell death in DU145 cells via the ERK signaling pathway. a Western blotting of ERK signaling-related protein expression levels in DU145 cells treated with indicated concentrations of SH003. b Western blotting of ERK2 and PARP levels in DU145 cells transfected with ERK2-expressing or control vectors and treated with indicated concentration of SH003 for 24 h. c Flow cytometry analysis of apoptotic cell death of DU145 cells transfected with the ERK2-expressing or control vectors. Results are presented as the means ± SD of three independent experiments. *P < 0.05 comparison of control vector-transfected cells with ERK2-transfected cells treated with SH003 in late apoptosis, #P < 0.05 comparison of ERK2-transfected cell with SH003 treatment in early apoptotic cells. d Schematic of the hypothetical system regulated by SH003. SH003 induces apoptosis in prostate cancer cells and inactivates ERK signaling