Fig. 2From: SH003 induces apoptosis of DU145 prostate cancer cells by inhibiting ERK-involved pathwayEffects of intracellular ROS generation on apoptotic cell death of DU145. a Cells were treated with 50, 250, or 500 μg/mL SH003 and H2DCF-DA dye for 1 h at 37 °C. b Cell were pretreated with 3 mM NAC for 30 min and then treated with 500 μg/mL of SH003. ROS generation in DU145 cells was measured by flow cytometry. Experiments were repeated three times. *P < 0.05. c Cells were pretreated with 3 mM of NAC for 1 h and then treated with 500 μg/mL SH003 for another 48 h. Apoptosis was detected by flow cytometry and data are presented as the means ± SD. Experiments were performed in triplicate. *P < 0.05. d Apoptosis-related protein levels were detected by western blottingBack to article page