Fig. 1

SH003 induces apoptosis in DU145 cells. a DU145 cells were exposed to the indicated concentrations of SH003 for 72 h or treated with 30% ethanol as control, and cell viability was measured by the MTT assay. Experiments were performed three times independently and data are presented as the means ± SD.*P < 0.05. b Cells were treated with SH003 for 48 h and double-stained with anti-BrdU and propidium iodide (PI). Cell proliferation was determined using cells stained with BrdU for DNA synthesis and DNA content was detected by PI. The dot blots display BrdU incorporation (Y-axis) and DNA content (X-axis). c DU145 cells were exposed to SH003 for 48 h and then stained with Annexin V and 7-AAD before flow cytometry. Data are presented as the means ± SD and experiments were replicated three times. *P < 0.05. d Levels of apoptosis-related proteins were detected by western blotting with indicated antibodies after treatment with SH003 for 24 h. β-actin was used as a loading control. Black triangle indicates increasing concentrations of SH003 (50, 250, and 500 μg/mL)