Fig. 7
From: Anti-aging and tyrosinase inhibition effects of Cassia fistula flower butanolic extract
Tyrosinase inhibition assay was determined in the C. fistula flower extract. Various concentrations of the flower extract were mixed with 100 U/mL of mushroom tyrosinase in a sodium buffer (pH 6.4). L-DOPA substrate (1 mM) was added into the reaction mixture and it was then incubated for 10 min. The change of the absorbance was measured at 490 using a microplate reader. All assays have been demonstrated in triplicate and the mean ± standard deviations are shown ****p < 0.0001 versus the control without the extract