Fig. 6
From: Anti-aging and tyrosinase inhibition effects of Cassia fistula flower butanolic extract
The effects of C. fistula flower extract on HA synthesis were determined using an ELISA kit. Fibroblast cells were pre-treated in serum free medium for 6 h and were further treated with or without various concentrations of the flower extract (0–200 μg/mL) for 48 h. The cultured medium was collected for ELISA and the absorbance was measured at 450 and 570 nm using a microplate reader. All assays have been performed in triplicate and the mean ± standard deviations are shown as **p < 0.01, ***p < 0.001 and ****p < 0.0001 versus the non-treated cells