Antinociception of petroleum ether fraction derived from crude methanol extract of Melastoma malabathricum leaves and its possible mechanisms of action in animal models

Table 4 Antinociceptive activity of PEMM assessed by the hot plate test in mice

Treatment	Dose (mg/kg)	Latency of discomfort(s) at respective time interval (min)
Treatment	Dose (mg/kg)	0 min	60 min	90 min	120 min	150 min	180 min	210 min
10% DMSO	-	6.97 ± 0.22	6.97 ± 022	6.90 ± 0.23	6.15 ± 0.15	6.92 ± 0.23	6.88 ± 0.29	6.35 ± 0.17
Morphine	5	5.77 ± 0.15	17.37 ± 1.03^a	18.25 ± 0.74^a	16.52 ± 1.22^a	13.67 ± 1.43^a	11.22 ± 1.11^a	10.48 ± 0.58^a
PEMM	100	6.89 ± 0.11	9.43 ± 0.36^a	9.67 ± 0.22^a	7.68 ± 0.35	7.36 ± 0.20	7.51 ± 0.17	6.95 ± 0.20
	250	6.36 ± 0.27	9.60 ± 0.24^ab	10.30 ± 0.30^ab	10.41 ± 0.60^ab	9.82 ± 0.44^ab	9.22 ± 0.20^a	7.97 ± 0.20^a
	500	6.36 ± 0.08	11.92 ± 0.91^ab	13.40 ± 0.48^ab	13.38 ± 0.56^ab	12.43 ± 0.91^a	10.33 ± 0.27^a	9.13 ± 0.08^a
Naloxone (NLX)	5	6.38 ± 0.27	6.43 ± 0.41	5.98 ± 0.46	6.10 ± 0.21	5.93 ± 0.68	6.13 ± 0.58	5.67 ± 0.54
Naloxone + PEMM	5 + 500	6.71 ± 0.20	11.62 ± 0.72	13.40 ± 1.00	12.87 ± 1.00	12.00 ± 0.56	10.64 ± 0.27	8.88 ± 0.29

^aData differed significantly (P < 0.05) when compared against the control (10% DMSO-treated) group
^bData differed significantly (P < 0.05) when compared against the 5 mg/kg morphine-treated group

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